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KMID : 0380919750040010001
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Journal of the Korean Society of Food Nutrition
1975 Volume.4 No. 1 p.1 ~ p.6
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Determination of vitamin B₂ by the lumiflavin fluorometric method and FMN, FAD, FR by the paper chromatography in the feces and urine
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이현기/Lee HK
배송자/Bae SJ
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Abstract
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After ablactation, wistar strain white male rats, weighing 270g and 340g, were fed with a diet of CLEA for three months. The whole daily excretion of each feces and urine were collected, and extracted with water(80℃ hot water).
The combined extraction were filtered and the B₂ was determined with the parts of the filterates by the lumiflavin fluorometric method, and the FMN, FAD and FR with the rest of the filterates by paper chromatography.
The following results were obtained ;
1. B₂ contents in the feces were 27.52 γ per 100 grams per body weight, and 83.93 γ per each rat per day.
2. B₂ contents in the urine were 18.47 γ per 100 grams per body weight, and 56.33 γ per each rat per day.
The total daily excretion of B₂ contents in the feces were 1. 5 times as much as in the urine.
3. Among the total daily B₂ excretion of one white wistar strain rat in the feces were the following ; FAD, 81.0% ; FMN, 14.9% ; FR, 3.3%.
Therefore the order of the contents were FAD>FMN>FR.
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